A Novel Dehydration-Responsive Element-Binding Protein

PlantMolBiolRep(2010)28:664–675DOI10.1007/s11105-010-0196-y

ANovelDehydration-ResponsiveElement-BindingProteinfromCaraganakorshinskiiIsInvolvedintheResponsetoMultipleAbioticStressesandEnhancesStressTolerance柠条乮极强耐旱性的多年生灌木乯

涉及丆包含丆牵涉进

非生物体的丆无生命的

耐受性

inTransgenic转基因的

Tobacco

烟草

XueminWang&JieDong&YunLiu&HongwenGao

Published#online:19March2010Springer-Verlag2010

AbstractThedehydration-responsive脱水丆干燥

元素丆要素丆自然环境

element-binding(DREB)proteinspecificallybindsthedehydration-responsiveelementandispartofaplant-specificfamilyoftranscriptionfactorsthatplayimportantrolesinregulatingtheexpressionofgenesinresponsetoavarietyofabioticstresses,includingdrought,highsalt,andlowtemperature.Inthisstudy,aDREB-likegenecontainingaconservedAPETLA2(AP2)/ethyleneresponsiveelementbindingfactor(ERF)domain,termedDREbindingfactor(CkDBF),wasisolatedfromCaraganakorshinskii.TheK,R-richmotifintheN-terminalregionwasshowntoplayanessentialroleinnuclearlocalizationofCkDBF.DNA-bindingspecificityandtransactivationactivityofCkDBFwereverifiedbyyeastone-hybridexperiments.ExpressionofCkDBFwasshowntobeupregulatedbyhighsalt,dehydration,lowtemperature,andthephytohormoneabscisicacid(ABA).OverexpressionofCkDBFintransgenictobaccoplantsresultedinhighertolerancetohighsalinityandosmoticstressesandinductionofadownstreamtargetgeneundernormalconditions.TheseresultssuggestthatCkDBFmayplayanessentialroleasaDREBtranscriptionfactorinregulationofstress-responsivesignalinginC.korshinskii.

Keywords.Caraganakorshinskii.DREB.SignalingregulationStresstolerance

X.WangJ.DongH.Gao(*)InstituteofAnimalScience,

ChineseAcademyofAgriculturalScience,2-Yuan-Ming-YuanWestRd,HaidianDistrict,Beijing100193,People’sRepublicofChinae-mail:gaohongwen@http://doc.xuehai.net

Y.Liu

InstituteofZoology,ChineseAcademyofSciences,XianshanNanxincun20#,

Beijing100093,People’sRepublicofChina

Introduction

Variousabioticstresses,includinglowtemperature,waterdeficit,andsoilsalinization,heavilyinfluenceplantgrowthandcropproductivity(Boyer1982).Duringlong-termevolution,plantshavedevelopedcomplexmolecularmechanismstosurviveharshenvironments.Transcriptionfactor(trans-factor)genesplayimportantrolesinstresssurvivalbyservingasmasterregulatorsofsetsofdownstreamstress-responsivegenes.Transcriptionfactorsregulatedownstreamgeneexpressionviabindingtospecificelements(cis-elements)intargetgenes,andconsequently,enhancestresstoleranceinplants(ChenandZhu2004;Yamaguchi-ShinozakiandShinozaki2006).Severalhundredtypesoftranscriptionfactorshavebeenisolatedfromhigherplants.Importantfamiliesofstress-responsivetranscriptionfactorsincludetheethylene-responsiveelementbindingfactors(ERF),basic-domainleucine-zipper(bZIP)MYB(fromavianmyeloblastosisvirus),andWRKYtranscriptionfactors(Agarwaletal.2006;Liuetal.1999;Riechmannetal.2000).Thedehydration-responsiveelement-binding(DREB)proteinsmakeupasubfamilyoftheAP2/ERFtranscriptionfactorfamily(Magnanietal.2004;RiechmannandMeyerowitz1998).ThefirstisolatedDREBfamilymemberwasCRT/DRE-bindingfactor1(CBF1)(Stockingeretal.1997).Subsequently,twoDREBgenesregulatingdroughtandlow-temperature-responsivegeneexpressionwereisolatedfromArabidopsis(Liuetal.1998).Atpresent,manyDREBcomplementarydeoxyribonucleicacid(cDNAs)havebeenclonedandcharacterizedindifferentplants,includingrice(Oryzasativa)(Dubouzetetal.2003;Tianetal.2005),soybean(Glycinemax)(Lietal.2005),maize(Zeamays)(KizisandPages2002;Qinetal.2004),cotton(Gossypiumhirsutum)(HuangandLiu2006a,b),(Hordeum

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