紫叶李叶片色素含量及叶绿素荧光动力学参数对SO_2胁迫的响应

第28卷第11期

2008年11月

环　境　科　学　学　报　ActaScientiaeCircumstantiae

Vol.28,No.11Nov.,2008

李彦慧,孟庆瑞,李向应,等.2008.紫叶李叶片色素含量及叶绿素荧光动力学参数对SO2胁迫的响应[J].环境科学学报,28(11):2236-2242

LiYH,MengQR,LiXY,etal.2008.ContentsofphotosyntheticpigmentsandchlorophyllfluorescenceparametersinPrunuscerasiferavar.atropurealeavesunderSO2stress[J].ActaScientiaeCircumstantiae,28(11):2236-2242

紫叶李叶片色素含量及叶绿素荧光动力学参数对SO2

胁迫的响应

李彦慧,孟庆瑞,李向应,李保国,杨建民

1.河北农业大学,保定071000

2.乌兰察布职业学院生物技术系,集宁012000

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收稿日期:2007212210　　　修回日期:2008204218　　　录用日期:2008208205

摘要:以盆栽紫叶李的2年生嫁接苗为试材,采用人工静态熏气装置分析了不同浓度SO2(0～22.8mg m-3)胁迫条件下(16d)紫叶李叶片光合色素含量及荧光动力学参数的变化情况.研究结果显示:在SO2浓度为5.7mg m-3的胁迫下,叶绿素a(Chla)、叶绿素b(Chlb)、Chla/Chlb、总叶绿素和类胡萝卜素(Car)含量均比对照增加,在其它浓度处理下均减小,(11.4mg m-3a和类胡萝卜素除外).在SO2浓度小于11.4mg m-3时,(,p<0.01);而在SO2浓度大于11.4mg m-3时则随着胁迫强度的增强而降低(,<Fv/Fm随着胁迫强度的增强呈现先增加后降低的变化趋势,v/Fq,而荧光非光化学淬灭效率qN,S24mg m-3)胁迫下通过自身较强的保护系统使机体免受伤害;O2 mN耗散过剩的光能和提高ΦPSⅡ消耗多余能量来保护光合器官免受损伤,,紫叶李叶片受到伤害与PSⅡ反应中心失活有关.关键词:紫叶李;S2;光合色素;花青苷;荧光参数

文章编号:025322468(2008)1122236207　　　中图分类号:X171　　　文献标识码:A

ContentsofphotosyntheticpigmentsandchlorophyllfluorescenceparametersinPrunuscerasiferavar.atropurealeavesunderSO2stress

LIYanhui,MENGQingrui,LIXiangying,LIBaoguo,YANGJianmin

1.AgricultureUnversityofHebei,Baoding071000

2.BiotechnologyDepartment,WulancltabuVocationalCollege,Jining012000

Received10December2007;　　　receivedinrevisedform18April2008;　　　accepted5August2008

Abstract:Two2year2oldpot2plantedseedlingsofPrunuscerasiferavar.atropureawerestudiedtodeterminetheeffectofSO2stressonphotosyntheticpigments,anthocyaninandchlorophyllfluorescenceparameters.UnderSO2treatmentinaclosedchamberatarateof5.7mg m-3,thecontentsofchlorophylla,chlorophyllb,chlorophylla+chlorophyllb,carotenoidincreased,butthedifferenceswerenotsignificantcomparedwiththecontrol.HoweverforhigherlevelsofSO2,theseparametersdecreasedsignificantlycomparedwiththecontrol(exceptchlorophyllaandcarotenoidunder11.4mg m-3SO2stress).Asthestressincreased,thecontentofanthocyaninandflavonoidsincreasedatSO2concentrationsbelow11.4mg m-3,anddecreasedsignificantlyatconcentrationshigherthan11.4mg m-3.Consideringthechlorophyllfluorescenceparameters,Fv/FmfirstincreasedandthendecreasedastheSO2concentrationincreased,whileqNincreasedatgreaterSO2concentrations.Fv/FoandqPdecreasedaccordinglywiththeincreaseofSO2concentration.WeconcludethatPrunuscerasiferavar.atropureacouldprotectitselffromdamagethroughahighlyactiveprotectionsystemunderlowSO2concentrations(lowerthan11.4mg m-3),whileunderhigherSO2concentrations(higherthan11.4mg m-3)theprotectionmechanismcouldprotectthephotosyntheticapparatusfromdamageresultingfromsurpluslightenergythroughincreasingqNandΦPSⅡ,butthephotosyntheticpigmentsandtheotherchlorophyllfluorescenceparameterswerenegativelyaffected.TheresultsdemonstratethatdamagetotheleavesofPrunuscerasiferavar.

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基金项目:国家林业局“948”项目(No.2001240);河北农业大学科学研究基金项目

Supportedbytheprogramsof948oftheStateForestryAdministration(No.2001240)andtheScientificresearchofAgricultureUnversityofHebei

作者简介:李彦慧(1971—),女,副教授,E2mail:yanhuili01@163.com;3通讯作者(责任作者),E2mail:yangjm@hebau.edu.cn

Biography:LIYanhui(1971—),female,associateprofessor;E2mail:yanhuili01@163.com;3Correspondingauthor,E2mail:yangjm@hebau.edu.cn

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