三七叶_人参叶和西洋参叶皂苷类成分的研究_英文_张兰兰

23：1064-1068天然产物研究与开发NatProdResDev2011，

6880（2011）06-1064-05文章编号：1001-

三七叶、人参叶和西洋参叶皂苷类成分的研究

1，21*2222

张兰兰，高文远，周水平，朱永宏，宋兆辉，黄芝娟

1

2天津大学药学院，天津300072；天津天士力集团研究院中药所，天津300410

摘要：三七叶、人参叶和西洋参叶其皂苷类成分相近，但专属性成分各异，皂苷类成分的分布比例也各不相

UV法测定上述皂苷成分的方法，同。本文建立了HPLC-经过方法学考察，各种皂苷成分精密度好、加样回收率方法可靠。11种皂苷成分总含量顺序为：西洋参叶＞人参叶＞三七叶；二醇组皂苷成分含量：西洋参叶＞三高，

七叶＞人参叶；三醇组皂苷成分含量：人参叶＞西洋参叶＞三七叶。西洋参叶中二醇组皂苷和人参叶中三醇组皂苷含量明显高于其他。西洋参叶中人参皂苷Rb3和Rd的含量之和占11种皂苷成分的60%以上。鉴于其中人参皂苷的高含量，三七叶、人参叶和西洋参叶应该作为皂苷来源得到充分利用；不同的皂苷成分有不同的药理活性，应基于它们的皂苷组成和比例选择性进行研究和开发。关键词：三七；人参；西洋参；叶；人参皂苷中图分类号：R284.1

文献标识码：A

GinsenosidesinLeavesofPanaxnotoginseng，P．ginsengandP．quinquefolius

2

ZHANGLan-lan1，，GAOWen-yuan1*，ZHOUShui-ping2，ZHUYong-hong2，SONGZhao-hui2，HUANGZhi-juan2

1

CollegeofPharmaceutical＆Biotechnology，TianjinUniversity，Tianjin300072，China；

2

ModernTCMR＆DInstitute，TianjinTaslyGroupCo．Ltd．，Tianjin300410，China

Abstract：LeavesofPanaxnotoginseng，P．ginsengandP．quinquefoliushavesimilarsaponincomponentsbutwithdiffer-entexclusivecomponents．Inthiswork，thecontentsofsaponinsintheleavesofPanaxnotoginseng，P．ginsengandP．phasehigh-performanceliquidchromatography．Thechromatographicsepa-quinquefoliusweredeterminedwithreversed-rationwasachievedbyusingaKinetex-C18column（100×4．6mm，2．6μm，phenomenex，at30℃）．Elevencompoundswereseparatedwithagradientelutionanddetectedat203nm．Theresultsshowedthattheorderoftotalcontentofsapo-nins，protopanaxadiolsaponinsandprotopanaxatriolsaponinsinthreekindsofleaveswasP．quinquefolius＞P．ginseng＞P．notoginseng，P．quinquefolius＞P．notoginseng＞P．ginseng，andP．ginseng＞P．quinquefolius＞P．notogin-respectively．ThecontentofprotopanaxadiolsaponinsintheleavesofP．quinquefoliusandthecontentofproto-seng，

panaxatriolsaponinsintheleavesofP．ginsengobviouslywerehigherthanthoseinothers．AndthetotalcontentofGin-senosidesRb3andRdwasaccountedforover60%ofthetotal11ginsenosidesinP．quinquefoliusleaves．Conclusively，theleavesofPanaxnotoginseng，P．ginsengandP．quinquefoliusshouldbewellusedasawonderfulsourceforsaponinsallthethreekindsofsaponinsshouldbeexploitedonthebasisoftheircontentsduetotheirhighcontents．Inaddition，andcompositions．

Keywords：Panaxnotoginseng；P．ginseng；P．quinquefolius；leaves；ginsenoside

Instruction

Panaxginseng，P．notoginsengandP．quinquefoliusarevaluableagriculturecommodityusedinmanytradition-almedicinaltherapies．Inthepastyears，muchattentionhasbeenpaidtoanalyzetheactivesaponinsintheroot

ReceivedJanuary6，2010；AcceptedJune12，2010

*CorrespondingauthorTel：86-22-87401895；E-mail：pharmgao@tju．

edu．cn

ofP．ginseng，P．notoginsengandP．quinquefolius．Forexample，P．notoginsenghasbeenusedascreditablehaemostaticanddrugforthetreatmentofhaemoptysis，

haematomainChinaformorethan400years．ModernpharmacologicalstudieshaveshownthatP．notoginsenghasanticarcinogenicandhepatoprotectiveactivities，aswellasprotectiveeffectsoncardiovascularandcerebro-vascularsystems［1］．P．ginsenghasbeenatonic，seda-tive，anti-fatiguedrug，andalsohasanti-diabeticandanti-tumoractivities［2］．P．quinquefolius，whichisa

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